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nr recombinant human neuregulin 3 r d systems  (R&D Systems)


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    R&D Systems nr recombinant human neuregulin 3 r d systems
    Nr Recombinant Human Neuregulin 3 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+nrg1/Recombinant+Human+NRG1%2FHRG1+Protein%2C+CF/10__1016_slash_j__cpblue__2026__100015-232-157-161
    Average 93 stars, based on 40 article reviews
    nr recombinant human neuregulin 3 r d systems - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Passaging:

    Article Title: Intestinal tuft cell subtypes represent successive stages of maturation driven by crypt-villus signaling gradients.
    Article Snippet: In general, tuft cell specification was induced on the 3rd day after passaging with ENR medium supplemented with recombinant murine IL-4 and IL-13 (10 ng/ml each, Immunotools). .. Thereafter, tuft cell maturation was facilitated by removal of EGF, Noggin and R-spondin from the medium and addition of one of the following or a combination thereof on the 4th day after passaging: recombinant murine IL-25 (20 ng/ml, Immunotools), recombinant human BMP2 (20 ng/ml, Immunotools), recombinant human BMP4 (20 ng/ml, Immunotools), recombinant human NRG1 (20 ng/ml, R&D systems), recombinant murine WNT5a (20 ng/ml, Biotechne) and acetylcholine chloride (100μM, Sigma Aldrich). .. For investigation of alternative tuft cell inducing factors (Supplementary Fig. 6a, b), DAPT (5μM Sigma D5942) and Wnt-surrogate FC fusion protein (0.5 nM, U-Protein Express) were used.

    Article Title: Intestinal tuft cell subtypes represent successive stages of maturation driven by crypt-villus signaling gradients
    Article Snippet: In general, tuft cell specification was induced on the 3 rd day after passaging with ENR medium supplemented with recombinant murine IL-4 and IL-13 (10 ng/ml each, Immunotools). .. Thereafter, tuft cell maturation was facilitated by removal of EGF, Noggin and R-spondin from the medium and addition of one of the following or a combination thereof on the 4 th day after passaging: recombinant murine IL-25 (20 ng/ml, Immunotools), recombinant human BMP2 (20 ng/ml, Immunotools), recombinant human BMP4 (20 ng/ml, Immunotools), recombinant human NRG1 (20 ng/ml, R&D systems), recombinant murine WNT5a (20 ng/ml, Biotechne) and acetylcholine chloride (100 μM, Sigma Aldrich). .. For investigation of alternative tuft cell inducing factors (Supplementary Fig. ), DAPT (5μM Sigma D5942) and Wnt-surrogate FC fusion protein (0.5 nM, U-Protein Express) were used.

    Article Title: Mature tuft cell phenotypes are sequentially expressed along the intestinal crypt-villus axis following cytokine-induced tuft cell hyperplasia
    Article Snippet: In general, tuft cell specification was induced on the 3 rd day after passaging with ENR medium supplemented with recombinant murine IL-4 and IL-13 (10 ng/ml each, Immunotools). .. Thereafter, tuft cell maturation was facilitated by removal of EGF, Noggin and R-spondin from the medium and addition of one of the following or a combination thereof on the 4 th day after passaging: recombinant murine IL-25 (20 ng/ml, Immunotools), recombinant human BMP2 (20 ng/ml, Immunotools), recombinant human BMP4 (20 ng/ml, Immunotools), recombinant human NRG1 (20 ng/ml, R&D systems), recombinant murine WNT5a (20 ng/ml, Biotechne) and acetylcholine chloride (100 μM, Sigma Aldrich). ..

    Recombinant:

    Article Title: Intestinal tuft cell subtypes represent successive stages of maturation driven by crypt-villus signaling gradients.
    Article Snippet: In general, tuft cell specification was induced on the 3rd day after passaging with ENR medium supplemented with recombinant murine IL-4 and IL-13 (10 ng/ml each, Immunotools). .. Thereafter, tuft cell maturation was facilitated by removal of EGF, Noggin and R-spondin from the medium and addition of one of the following or a combination thereof on the 4th day after passaging: recombinant murine IL-25 (20 ng/ml, Immunotools), recombinant human BMP2 (20 ng/ml, Immunotools), recombinant human BMP4 (20 ng/ml, Immunotools), recombinant human NRG1 (20 ng/ml, R&D systems), recombinant murine WNT5a (20 ng/ml, Biotechne) and acetylcholine chloride (100μM, Sigma Aldrich). .. For investigation of alternative tuft cell inducing factors (Supplementary Fig. 6a, b), DAPT (5μM Sigma D5942) and Wnt-surrogate FC fusion protein (0.5 nM, U-Protein Express) were used.

    Article Title: Intestinal tuft cell subtypes represent successive stages of maturation driven by crypt-villus signaling gradients
    Article Snippet: In general, tuft cell specification was induced on the 3 rd day after passaging with ENR medium supplemented with recombinant murine IL-4 and IL-13 (10 ng/ml each, Immunotools). .. Thereafter, tuft cell maturation was facilitated by removal of EGF, Noggin and R-spondin from the medium and addition of one of the following or a combination thereof on the 4 th day after passaging: recombinant murine IL-25 (20 ng/ml, Immunotools), recombinant human BMP2 (20 ng/ml, Immunotools), recombinant human BMP4 (20 ng/ml, Immunotools), recombinant human NRG1 (20 ng/ml, R&D systems), recombinant murine WNT5a (20 ng/ml, Biotechne) and acetylcholine chloride (100 μM, Sigma Aldrich). .. For investigation of alternative tuft cell inducing factors (Supplementary Fig. ), DAPT (5μM Sigma D5942) and Wnt-surrogate FC fusion protein (0.5 nM, U-Protein Express) were used.

    Article Title: Phase separation of Hippo signalling complexes
    Article Snippet: .. For NRG1‐treated organoids, recombinant human NRG1 (100 ng/ml, R&D Systems) was added to complete IntestiCult Medium from day 0 of culture (for analysis of organoid growth) or 1 h before fixing (for acute stimulation). ..

    Article Title: Phase separation of Hippo signalling complexes.
    Article Snippet: .. For NRG1-treated organoids, recombinant human NRG1 (100 ng/ml, R&D Systems) was added to complete IntestiCult Medium from day 0 of culture (for analysis of organoid growth) or 1 h before fixing (for acute stimulation). ..

    Article Title: ADAM Metallopeptidase domain 19 promotes skin fibrosis in systemic sclerosis via neuregulin-1
    Article Snippet: .. HC primary dermal fibroblasts were subjected to a 24 h serum starvation, followed by adding 50 ng/ml recombinant human NRG1 (396-HB-050, R&D Systems, USA) for an additional 24 h. ..

    Article Title: An integrated transcriptomic cell atlas of human endoderm-derived organoids
    Article Snippet: .. Human colon organoids were maintained for 4 days starting from mechanically dissociated fragments in Matrigel (Corning, 356231) or Matrigel supplemented with 20% Collagen I (Advanced Biomatrix, 5225) in Advanced DMEM/F12 with penicillin/streptomycin, 1× Glutamax and 10 mM HEPES, supplemented with 1× B27 supplement (Gibco), 1 μM N-acetylcysteine (Sigma-Aldrich), 0.5 nM Wnt Surrogate-Fc Fusion Protein (U-Protein Express B.V., N001), 100 ng/ml Noggin (EPFL Protein Expression Core Facility), 500 ng/ml R-Spondin 1 (EPFL Protein Expression Core Facility), 100 ng/ml recombinant human IGF-1 (BioLegend), 50 ng/ml recombinant human FGF-2 (Peprotech), 10 nM gastrin, 100 ng/ml recombinant human NRG1 (RD, 5898-NR) and 500 nM A83-01 (Tocris). .. Human mini-colons were maintained for 14 and 21 days, where starting from day 7 apical medium was switched to ‘ENR’ composed of 50 ng/ml recombinant human EGF (RD, 236-EG), 100 ng/ml Noggin (EPFL Protein Expression Core Facility), 500 ng/ml R-Spondin 1 (EPFL Protein Expression Core Facility), and organoid medium as described above was maintained on the basal side.

    Article Title: ADAM Metallopeptidase domain 19 promotes skin fibrosis in systemic sclerosis via neuregulin-1.
    Article Snippet: .. Fibroblast treatment with recombinant human NRG1 HC primary dermal fibroblasts were subjected to a 24 h serum starvation, followed by adding 50 ng/ml recombinant human NRG1 (396-HB-050, R&D Systems, USA) for an additional 24 h. ..

    Article Title: Mature tuft cell phenotypes are sequentially expressed along the intestinal crypt-villus axis following cytokine-induced tuft cell hyperplasia
    Article Snippet: In general, tuft cell specification was induced on the 3 rd day after passaging with ENR medium supplemented with recombinant murine IL-4 and IL-13 (10 ng/ml each, Immunotools). .. Thereafter, tuft cell maturation was facilitated by removal of EGF, Noggin and R-spondin from the medium and addition of one of the following or a combination thereof on the 4 th day after passaging: recombinant murine IL-25 (20 ng/ml, Immunotools), recombinant human BMP2 (20 ng/ml, Immunotools), recombinant human BMP4 (20 ng/ml, Immunotools), recombinant human NRG1 (20 ng/ml, R&D systems), recombinant murine WNT5a (20 ng/ml, Biotechne) and acetylcholine chloride (100 μM, Sigma Aldrich). ..

    Expressing:

    Article Title: An integrated transcriptomic cell atlas of human endoderm-derived organoids
    Article Snippet: .. Human colon organoids were maintained for 4 days starting from mechanically dissociated fragments in Matrigel (Corning, 356231) or Matrigel supplemented with 20% Collagen I (Advanced Biomatrix, 5225) in Advanced DMEM/F12 with penicillin/streptomycin, 1× Glutamax and 10 mM HEPES, supplemented with 1× B27 supplement (Gibco), 1 μM N-acetylcysteine (Sigma-Aldrich), 0.5 nM Wnt Surrogate-Fc Fusion Protein (U-Protein Express B.V., N001), 100 ng/ml Noggin (EPFL Protein Expression Core Facility), 500 ng/ml R-Spondin 1 (EPFL Protein Expression Core Facility), 100 ng/ml recombinant human IGF-1 (BioLegend), 50 ng/ml recombinant human FGF-2 (Peprotech), 10 nM gastrin, 100 ng/ml recombinant human NRG1 (RD, 5898-NR) and 500 nM A83-01 (Tocris). .. Human mini-colons were maintained for 14 and 21 days, where starting from day 7 apical medium was switched to ‘ENR’ composed of 50 ng/ml recombinant human EGF (RD, 236-EG), 100 ng/ml Noggin (EPFL Protein Expression Core Facility), 500 ng/ml R-Spondin 1 (EPFL Protein Expression Core Facility), and organoid medium as described above was maintained on the basal side.



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    Image Search Results


    AHR-activated macrophages impair trophoblast function. ( A ) Heatmap showing expression levels of CGAS , NLRP3 , and TLR9 in various cell types determined by scRNA-seq. ( B ) Bar plots displaying significantly enriched GO terms in EVTs from URPL pregnancies. ( C ) Schematic diagram illustrating the co-culture system. ( D ) Representative images western blot images of TLR9, NLRP3, and cGAS protein expression in primary EVTs co-cultured with KYN-pretreated dMφs or control dMφs (n = 3 per group). ( E ) Representative immunofluorescence images showing co-localization of HLA-G and cGAS in hTSCs-EVTs co-cultured with KYN-pretreated dMφs or control dMφs (n = 5 per group). ( F ) Flow cytometry analysis of apoptosis levels in primary EVTs following co-culture with dMφs (n = 3 per group). ( G ) Scratch wound healing assay evaluating the migration capacity of HTR-8/SVneo cells (n = 3 per group). ( H ) Transwell invasion assay measuring the invasive ability of HTR-8/SVneo cells (n = 3 per group). ( I ) Western blot analysis of proteins associated with NF-κB signaling (p-NF-κB p65, NF-κB p65, p-IκBα, IκBα), apoptosis (BCL2, cleaved Caspase-3), and extracellular matrix remodeling (MMP9, MMP2) in HTR-8/SVneo cells. Data are presented as mean ± SD. Statistical significance was determined using the Student's t-test for two-group comparisons and one-way ANOVA for multiple comparisons; ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: International Journal of Biological Sciences

    Article Title: Excessive Kynurenine Metabolism Impairs Lysosomal acidification and Triggers mtDNA Release via the AHR/CISH/ATP6V1A Axis in Decidual Macrophages Associated with Unexplained Recurrent Pregnancy Loss

    doi: 10.7150/ijbs.121947

    Figure Lengend Snippet: AHR-activated macrophages impair trophoblast function. ( A ) Heatmap showing expression levels of CGAS , NLRP3 , and TLR9 in various cell types determined by scRNA-seq. ( B ) Bar plots displaying significantly enriched GO terms in EVTs from URPL pregnancies. ( C ) Schematic diagram illustrating the co-culture system. ( D ) Representative images western blot images of TLR9, NLRP3, and cGAS protein expression in primary EVTs co-cultured with KYN-pretreated dMφs or control dMφs (n = 3 per group). ( E ) Representative immunofluorescence images showing co-localization of HLA-G and cGAS in hTSCs-EVTs co-cultured with KYN-pretreated dMφs or control dMφs (n = 5 per group). ( F ) Flow cytometry analysis of apoptosis levels in primary EVTs following co-culture with dMφs (n = 3 per group). ( G ) Scratch wound healing assay evaluating the migration capacity of HTR-8/SVneo cells (n = 3 per group). ( H ) Transwell invasion assay measuring the invasive ability of HTR-8/SVneo cells (n = 3 per group). ( I ) Western blot analysis of proteins associated with NF-κB signaling (p-NF-κB p65, NF-κB p65, p-IκBα, IκBα), apoptosis (BCL2, cleaved Caspase-3), and extracellular matrix remodeling (MMP9, MMP2) in HTR-8/SVneo cells. Data are presented as mean ± SD. Statistical significance was determined using the Student's t-test for two-group comparisons and one-way ANOVA for multiple comparisons; ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: To induce hTSCs-derived EVTs (hTSCs-EVTs), hTSCs were seeded onto 6-well plates pre-coated with 1 μg/ml collagen IV and cultured in 2 mL of EVTs medium [DMEM/F12 supplemented with 0.1 mM 2-mercaptoethanol, 0.5% PS, 0.3% BSA, 1% ITS-X, 100 ng/ml NRG1 (CST, Cat#26941), 7.5 μM A83-01, 2.5 μM Y27632, and 4% KnockOut Serum Replacement (KSR, ThermoFisher, Cat#10828010)].

    Techniques: Expressing, Co-Culture Assay, Western Blot, Cell Culture, Control, Immunofluorescence, Flow Cytometry, Wound Healing Assay, Migration, Transwell Invasion Assay